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July 2011 Waksman Pipetting quiz

Multiple Choice
Identify the choice that best completes the statement or answers the question.
 
 
Pipettor Practice:

nar001-1.jpg
 

 1. 

Which of the above pipettor should be used for pipetting 19ul into a microtube
a.
1
b.
2
c.
3
d.
4
e.
none of the above are appropriate choices
 

 2. 

Which of the following should be used for pipetting 183.4ul?
a.
1 only
b.
2 and 3
c.
3 only
d.
3 and 4
e.
4 only
 

 3. 

Assuming you can use one pipettor, when pipetting 218 ul you should NOT use which of the following pipettes?
a.
1
b.
1 and 2
c.
1 and 2 and 3
d.
1 and 2 and 3 and 4
e.
4 only
 

 4. 

Ruth Smith was labeling her clones for making an ON culture and was unsure of the correct labeling notation. Please choose the correct notation for her.
a.
17SR1.11
b.
17RS1.11
c.
17ruth1.11
d.
RS1701.11
e.
17.RS.1.11
 
 
Pipettor Window:

nar002-1.jpg

nar002-2.jpg
 

 5. 

Which pipettor would be ideal to pipette volume #5?
a.
1
b.
2
c.
3
d.
4
e.
none of the above
 

 6. 

Which of pipettor would be ideal for pipette volume #6
a.
1
b.
2
c.
3
d.
4
e.
none of the above
 

 7. 

Which of the following should NOT be used for 42 ul?
a.
1, 2, 3,
b.
1, 2, 4
c.
2, 3, 4
d.
1, 2
e.
1, 3, 4
 
 
Pipetting technique

The following table describes the process of pipetting.  Each letter and it’s column separate is a pipetting event. Remember, you start pipetting  (ready position) and depending on what you are trying to accomplish each column corresponds to a separate process.

nar003-1.jpg
 

 8. 

Which column corresponds to “expelling” your volume?
a.
A
b.
B
c.
C
d.
D
 

 9. 

Which of the following corresponds to the technique of  “pulling up” a volume into a pipettor?
a.
A only
b.
A then B
c.
C  then D
d.
C  only
 

 10. 

The following represents 2 uL of blue dye expelled from your pipettor on to filter paper.

mc010-1.jpg
Which using the above chart, which of the following answers is correct?
a.
The dot labeled 1 corresponds to technique A then B.
b.
The dot labeled 2 corresponds to technique A then B
c.
The dot labeled 4 corresponds to B then A
d.
The dot labeled 3 corresponds D then C
e.
All of the above are incorrect
 



 
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