Mr. Alfano's Schedule

Research Class Resources

Biology I/II Resources

RCSB Protein Data Bank

Proteopedia

Macromolecular Museum

BLAST Search

Pingry Journal Databases

Pingry MS Swim Team

Pingry Speech & Debate Team

Intro to scientific Research

Current Labs

Recombination Cloning Using pAMP and pKAN

Plasmid Map (pAMP & pKAN)

NEB Cutter

Quiagen MiniPrep Handbook

Quiagen-QIAquick DNA Cleanup

Mystery Plasmid Identification

Mystery Plasmids and Enzymes

NEB Plasmid Maps

Restriction Enzyme Activity Chart

Sample Mystery Plasmid Presentation

PCR Cloning and His-Tagging of Fluorescent Protein

zsGreen Coding Region (Text File)

pET 28a (+) Expression Vector Map

Qiagen MiniPrep Handbook

QiaQuick Spin Handbook (PCR Cleanup and Gel Extraction)

Affinity Purification and Analysis of His-Tagged Proteins

Analysis of Purified Protiens by SDS-Polyacrylamide Gel Electrophoresis

DNA Tutorials

DNA Structure and Function

Tutorial 1

Tutorial 2

Tutorial 3

RT PCR Tutorials

Applied Biosystems RT-PCR Basics

Microbiology and Immunology Online

Syber Green Stain

RT PCR Animation (In French)

Lab Equipment Manuals

NanoDrop Spectrophotometer

New England BioLabs Ladder Guide

MyCycler Thermal Cycler PCR Machine

Microinjector Syringe Filling Manual

Gene Delivery Prodedure for Microinjection

Microinjector Pipette Cookbook