December 1

Drew is requesting a report from each school to be received by us NO LATER THAN THURSDAY, DECEMBER 3, 2009. It should be sent electronically in an email and include:

1) your school's updated Combined Clone Report Sheet

2) the Power Point Slides of the mocked up gel pictures for all gels run so far

3) the Power Point Slides of the Clone Summary Presentation

Dec 2

Clones for Sequencing organized by David Sukhin and Caryn Ha

David and Ed gel of direct colony PCR restriction digests.  Waiting on Mocked up version????

Updated Clone Sheet  by M&M

Presentation ppt.

HOMEWORK:

Please review the above and DOUBLE CHECK  your results against what is listed here as the stated results. We need to be sure ALL version of the data MATCH!

So check

-clone sheet

-Clones for sequencing

-and go through ppt find all your data....be sure all is correct and correctly labeled!

Dec 3

Back to Evolution

Review the body of evidence of evolution

Be sure to unstand gene pool

& population

&

Microevolution

 Using the "fish" from your notes identify

 p & q

Reminder:

p+q=1- allelic freq, describes the population NOT individuals

p2 + 2pq+ q2=1 describes genotype or individuals in the population.

The information from one equation can be used to derive the information in the other....

for HW see if you can find the frequency of the

using the equation we can determine the frequency of alleles of a population

ADDITIONAL WORK WAKSMAN PROJECT

Within a partnership create 10 slide presentation that describes the entire Waksman Project to the point that we have completed. You can mention the intended outcome of the bioinformatic portion but you only need to cover that terms of clarification. After discussion with the bio teachers, I decided to have you present to the class and your peers will help grade the project (in addition to my grade) during your presentation.

Due the 16 17th 18th- all should be prepared to present by the 16th. 

Dec 4

Waksman Meeting at Rutgers

4 pm

Permission Slip

Leaving at 3:30 from Athletics Entrance

Confirmed attending;

Ed X. and Mich. Enn.,  Madi Taylo, Marisa Werner

5/6

Big Blue Invitational!

7

9

Evolution Continues to the Origin of Life.

Please read 16.1- 16.6

Please put Stanley Miller diagram in your notebook.

Review the significance of the pieces that make up the experiment.

What is the evidence against SM experiment?

Please look at the video posted to the left about the orgin of life.

12/13

Practice quiz

Practice quiz

Practic Review Quizzes from first semester. Please use you notes and submit by monday night /Tuesday am

14

Final Guidance on WSSP presentations:

Please remember that you need to include analyis of YOUR gels. Though many gels contain lots of students  work just circle YOUR work and be prepared to discuss your work  Don't hesistate to discuss results that you do not understand or that did result in what was expected.  What are the sizes of your all your clones?  How did you come to that knowledge.

Complete practice clone 1 and 2....at least part of 2

15

More on practice clones.

16

Presentations Begin for WSSP

ALL STUDENT SHOULD HAVE  Presentations complete by this class. The order of presentation will be random so be ready to present your work.

18

Last day of classes

Off to Chemistry!!!!

Monday Nov 2

SMART Team Field to Columbia University- Dr. Scott Banta

Movie: Judgement Day

Tuesday, Nov 3

Judgement Day Continues

Behe Website

Wed, Nov 4

Reviewed DNA extraction for PV92 Insert. Click here for lab instructions.

Evolution Discussion today about Darwin and the Origin of the Species. And evidence of for evolution- vestigial structures, homologous structures. Review ppt for hw.

Read Chapter 13 and make sure that you have reviewed the links.

Thursday, Nov 5

DNA extraction first period

Evolution Continues

Evolution questions due monday

Friday, Nov 6

Waksman quiz! Open notes.

Make sure that you have your notebook and gels annotated.

Diagram bank that will be used on the quiz.

9

Waksman

Overnights of new colony picks.

10

Restriction digests of ALL clones over 500 base pairs.

Careful notation on tubes

11

Run gel on PCR'ed colonies.

Some groups need to prepare PCR reactions.

I will post the gel for the second batch of colony picking as soon as I can. Please mock up and put in your notebook

Be prepared to give all clone data to Michaela tomorrow. She will record the data on her spread sheet..

12 Fall Play

Professional Development Conference

Evolution video 1st period/ answer question:

Evaluate the following statement: "the bacteria grew resistant  since the introduction of antibiotics". According to the principles of evolution, do you agree or disagree with this statement? Support your answer by incorporating your understanding of the terms diversity, fitness, and reproduction. (You DO NOT need to define these terms; make sure you use them properly in context of your discussion. Answer in no more than 4-5 sentences.

2nd period- Micheala will have the clone sheet and you should be giving the information about your clones. Remember it needs to be complete!!!!! ALL data, including all PCRs- even if the insert is under 500 bp needs to be recorded.

13 Fall Play

Professional Development

Conference

Evolution video

answer questions for hw.

Evaluate the following statement: "  the modern day whale evolved from modern day wolves". According to the principles of evolution, do you agree or disagree with this statement? Support your answer by incorporating your understanding of the terms diversity, fitness, and reproduction. (You DO NOT need to define these terms; make sure you use them properly in context of your discussion. Answer in no more than 4-5 sentences.

16

Give back papers!!

Went over grades

comments

Evolution questions from last week are being collected on Wed

18

Evolotion questions collected

Evolution

Review miniprep protocol. Be sure that you do!!!!

19

Mini-prep your Overnights that are over 500 base pairs-

please review how to complete minipreps. The sequencing of your clone depends on the quality of the miniprep.

You can- if you have time- set up your digest tubes WITHOUT adding enzyme. We will add the enzyme in class on friday

20

Set up restriction digests.

at CP  please come back and add loading dye to your digest.

put  your digest inthe correct box.

23

Gel of restriction digest of student selected clones of direct colony PCR. 

25 Thanksgiving Break

30

Preparation for the waksman meeting.

Assignments were divided among the class.

All ppt are due on Wednesday.