Monday

Life on Earth Article by Ricardo and Szotstak

Please read this article and review the hurdles necessary for life to get started.

Atmosphere?

Molecules necessary for life?

Replication?

Polymerization?

COMPLETE YOUR CLONES!

Tuesday

FRIDAY SCHEDULE:

Period 2/7 Lab

Period2:

Looking in your water? 1 period only. Is there a difference in the number of and type of organisms that you are finding now?

Discussion of article.

What was the article about?

 What are the significant points that the article highlights in the search for the origin of life?

What are the missing ideas from the article?

Reading: 294-297

Reading  318-319

COMPLETE YOUR CLONES!

Wednesday December 1

Stanley Miller

Hypothesis

 Prebiotic conditions for life

Origins of eukaryotes.

Assignment:

Evolution cartoon Project

www.pixton.com

Working in groups of three to four students, you will be required to make a cartoon related to the topic of evolution using the software at the following link: www.Pixton.com.   You will be required to illustrate the following 3 scenarios or ideas in your comic strip.

The Pingry Robotics Team has built a time machine and is planning to send several Biology II students back in time.  The mission is to provide insight in the biology occurring at various time points visited on the prehistoric earth:

A.      Your first stop is 4 billion years ago where you collect primordial soup containing the precursors to life.  What are the conditions like? You observe a polymer in this soup that can copy itself. Identify this molecule.

B.      Your next stop is 3 billion years ago where you find organisms that produce glucose from CO2 using “cyclic photophosphorylation”.  An eager colleague runs through the airlock to collect samples but quickly dies and turns blue. Explain why this man suffocated in the presence of photosynthetic organisms.

C. Your next stop is 1 billion years ago where you find organisms similar to today’s plants that produce glucose from CO2 and release O2 but these organisms also obtain energy from the glucose they synthesize.  These aerobic organisms generate 18 times more energy from glucose as compared to the organisms from part B. How is the extra energy generated?

Due next thursday

Complete your clones!

2 Thursday

 Origin of Life and

Origins of Eukaryotes

Endosymbiosis

3 Friday

Worked on Practice Clone 4

Due today.

6

Charles Darwin

And the body of evidence for evolution

Read: 258-263

Which embyo?

7

How do populations evolve? What are the raw ingredients for change within a species.

Vocabulary:

Population

gene pool

genetic variation

mutation

sexual reproduction

Read: 264-267

Answer the following in your notes:

What does meiosis and variation and evolution have to do with each other?

Hardy Weinberg worksheets.

Is the Pingry School in Hardy Weinberg equilibrium?

8

Special Schedule- musicians report for second period  Assembly: Festival

9  Friday Schedule Today!  

Cartoons due!

14 In class quiz/ Take home quiz on evolution- with short answer essay question 22 points

due tomorrow

16  Semester Exam w ith FKF and Torres groups

PUT answers online for semester exam between 3 and 10 pm.

17 Last Day of Classes for BIOLOGY!

On to Chemistry in January!

1  Monday

Finishing Genetics

Problem set 2 collected today.

2 Tuesday

Check point survery distributed in class and to be collected before the end of class.

Biological Case Study to be completed in small groups of three to four. One person should act as secretary to record group answers. To be collected in class on Thursday.

5 Friday

PCR worksheet and

quiz on PV 92

SMART Team Field Trip to D.E. Shaw, NYC

6/7 Saturday/ Sunday

Midsemester test link here. (link removed) You are allowed to work other people to discuss the questions BUT not share answer- debate, compare, share ideas, but not you final choice. Due thursday November 11.  Two submissions will take the average of the two submissions.

8 Work on Test tonight

Look at gel results

Section 3/2 gel did not get saved!!! And it was good! All of you got DNA! But I am missing the image somehow.

9

NEW VERSION OF THE TEST HERE! VERSION 2. Please use this test. I will not grade the first version!

2hiu 2hiu 2hiu 2hiu 2hiu 2hiu

10

Comments are due from your teachers to upper school office.

Redo quiz on PV 92

 Open notes

11

test deadline extended to Friday

Soccer game

12

STRESS DOWN DAY and a DRESS DOWN DAY!

Test deadline at midnight.- if you need an extension please let me know.

Going outside to collect water samples!

Thinking about evolution.

Covered the golden rules of evolution and video introducting evolutionary theory

15

Section 1/2 HW" Please finish through the Blast X of PC3

Section 3/4 & 5/6 be ready with PC 3 downloaded and FInchTCV open an ready to work in class on Tuesday.

Bring Computers to Class and download practice clone 3!

We will be working through practice clone 3

Waksman

16

Went over the Test!

Waksman

17

Blast N

BLast X

Blast P

ppt from class

Waksman

18

Bring computers...be ready to work on Practice clone 3!

Waksman continues!

19

Finish Practice Clone 3

THose who are finished should go on to  PC4

Bring Computers to work on Practice Clone 3!

Learning and the Brain Conference (Boston)

Waksman

Practice clone 4 is due

22

Parent Conferences

23

Professional Development Day

No Classes

24

Thanksgiving Break

25

Thanksgiving Day

26

Thanksgiving Break

 Take home quiz

4

Take home quiz due

Back to Waksmans StudentScholars Project

Review of project

Review of  Protocols and instructions for

Picking colony

ON

PCR

Running a gel

New techniques

MINI-prep of the plasmid

Restriction digest

Running a gel

1)Some student will pick additional clones due to contamination in PCR

2)Some students will run another PCR reaction to confirm their PCR results

3) Some students will run a restriction digest on acceptable clones.

We will plot out your individual path for your clone this week.

HW: miniprep and restriction digest into notebook, on folded page. You can abbreviate terms but you need to keep the important ideas.

5

Review of  Protocols and instructions

Small groups of of overlapping interests assigned

Independent work

Section 2/7- refreshed overnights

Drew and Lisa Picked again- made ON- updated google docs

HW- Review miniprep protocol

Section 3/4

Section 5/6

Miniprep- protocol sheet here

6

Independent Work

MINI PREPS

DONE!

Please complete the 5 minute spin activity for HW (about graphing a cell going through mitosis)

7

 Independent Work

Restriction Digest of your plasmid. 

8

Make Uncut plasmid dilutions.

Run Gels of your digest.

Bridges Run

9/10 Parents Weekend

12 Understanding

Practic Clones

GELS

Section 2/7

Section 3/4 1st gel

Section 3/4 2nd gel

Section 5/6 1st gel

Section 5/6 2nd gel

DSAP link here

13

PSAT's

Finish analyzing the gels

Click here for ppt on your gel.

Please be sure your annotation is correct on your gel. I need to send them to Rutgers asap.

14

Practice Clone and DSAP

15 Clone list needs to be completed to be sent to RUTGERS today

Emily Kamen, Benito, ADeak, Rahul, Becca gel

Please see me for the order of the lanes. Andrew is all the way to the right.

Please do the practice tuturial on DSAP. Look for the instructions on the log in page.

Waksman Meeting

Leaving at 4 pm returning at 6:30 pm

18

Back to Genetics

Movie: Lorenzo's Oil

See links about Lorenzo's Oil to right

HW link here, questions to be answered for Wed.

Find and choose a genetic disorder to research

Genetics Project:

A Pamphlet on a particular inherited genetic disorder. You will be responsible for creating a professional pamplet on your given disease.  You will need to create your pamphlet for the parent who is facing a newly diagnosed child. You will need the background of the disease, its inheritance, and its mechanism of action, you should include symptoms, treatments and how to manage the disease. You should include at least three support organizations that are helpful for this parent.  Diagrams and images should be present to balance text, though you need to avoid large blank areas.

19

Be prepared to state your genetic disorder- try to avoid overlap with classmates

Finish Lorenzo's Oil

Genetics and Inheritance  continues

HW about ALD page is due tomorrow

PRACTICE CLONES 1 and 2 should be  attempted

20

Lorenzo's Oil HW due

Final list of chosen disorders due

ALD HW collected

Genetics Introduction

Go over pamphlet rubric and project

List of genetic disorders.

HW:  READ: 9.1-9.6

ppt

21

More genetics

Di hybrid cross and understanding  prediction versus actual numbers.

Testcross.

Complete genetics Problems sheet 1 for HW.

Work on pamphlet.

22

Work on pamphlet

More types of Inheritance

25 No Classes.

NJAIS- Lawrenceville School

26

Sex Linked inheritance

Sex Linked Crosses

Sex Determination

READ 9.12-9.16

9.23-9.24

Work on pamphlet

Work on genetics problem set 2. Handed  out in class.

PRACTICE CLONES????

27

Go over

1. Genetics Problems that were assigned for HW. If you were absent yest. Please see me in the AM  to get a copy.

Pedigree Concept

Read 9.19 and 9.20

PRACTICE CLONES????

28

Pamphlet Due for Genetic Disorder

PRACTICE CLONES?????

Recovering the Romanovs Link. This is an interesting story on Anastasia Romanov and the story of the coup that occurred in Russia that resulted in the killing of the Romanov Family. It's a click through interactive activity involving genetics, pedigrees and of course murder.

29

Community Service Day

Rufus Gunther Day

9 School Closed

 Rosh Hashanah

10 Introduction to Waksman Student Scholars Program and duckweed.

What is interesting about Duckweed? How can we study these genes? How can you create a cDNA library? Why are we interested in having a cDNA library and  not a genomic library?

Lots of review vocab!

ORF, UTR, reading frame, codon! Transcription and translation.

Introduction ppt

13

Convocation

Special Dress Code

Picking colonies from the library

Sterile technique

Labeling Clones

Making Overnights

ppt

14

Picking colonies

for overnights

2ml in culture tube

and PCR discussion

HW- review PCR! What is required for a PCR reaction

ppt

15 Setting up and running PCR

WSSP September 2010 PCR of our OV

Homework:

Complete the pre-test on the DSAP program.

Link here

Complete the pretest tonight!

The pretest does not count for anything but it helps the scientists at Rutgers.

16

Setting up your PCR reactions.

Ran PCR on first clones

17

Ran gel

1% agarose gel

Gel pictures:

Section 2_7

Section 3_4

Section 5_6

Ladder annotation slide

HW

Analyse results of your gel. Remember the PCR primers add about 200 nucleotides to the length of your cDNA insert.. To get the final size of the insert you need to substract off 200 bases from your PCR fragment length.

You must put the slide in a ppt and correctly annotate your insert size and the ladder. See the ladder annotation slide link abovel

Take home quiz. 20 points.

No afterschool activities

18/19

Yom Kippur

Mrs. O'Mara's 70.3!!!!

20 Annotation of gels due\

Quizzes collected

21 Annotation of gels do again !

Quizzes reviewed

22 Annotation due of gels AGAIN!!!!

Please get it right this time! grrrr.

Starting Mitosis/ Cell Cycle and clicker questions BEGIN this week!!!

Read the following sections in your textbook:

8.3 Prokaryotic reproduction

8.4 Division of eukaryotic chromosomes

8.5 Cell cycle

8.6 Cell Changes (images)

8.12- chromosomes

Lots of terms today with vocab.

September 2010- Cell Division Mitosis Cell cycle cancer

24

Covered Mitosis and the phases of mitosis

In labs we observed phases of mitosis

Please review the links above for HW.

27

What controls Cell division?

What is cancer?

Read 8.8 Growth factor

density dependent Inhibition

anchorage dependence

What is cancer?

Types of cancer

28

Meiosis

A reduction division to form gametes

2n to 1n

diploid to haploid

Read 8.14- 8. 15

8.16, 8.17, 8.18,

Crossing over

Independent assortment

Genetic recombination

Watch Videos on links section

(upper right of this page)

Complete reading

Meosis ppt

Class trips begin

Form V and VI Depart 3 p.m.

29

No classes due to field trips.

Form V and  VI return

30  NO LAB DUE TO TRIPS THIS WEEK!

Meosis continues

Nondisjuction of Chromosomes during Meiosis

Down Syndrome or Trisomy 21

Abnormalities of sex chromosomes

Examples of polyploidy.

Ppt on Trisomies and Nondisjuction

Oct 1

Review Nondisjunction and associated syndrome of somatic non disjunctions and sex chromosome nondisjuctions- Trisomy 21, 13, 18 vs Turner Syndrome, Klinefelter Syndrome, XXX, XXy

FInished Chromosomal structural abnormalities

Duplication, inversion, deletion, translocation

See yest ppt for review

Take Home Quiz!

quiz! Mitosis, Cell Cycle, and Cancer and Meiosis, Nondisjuction- due Monday. You cannot share the quiz with anyone BUT you can discuss the concepts.